Total RNA purification is a fundamental step in numerous molecular biology studies. This process requires the careful retrieval of total RNA from diverse biological samples, such as tissues, cells, and body fluids. The objective is to obtain high-quality RNA that is uncontaminated from contaminating compounds. A variety of methods are available fo

RNA is a fragile molecule that is easily degraded by enzymes called RNases. Therefore, it is essential to meticulously handle RNA samples and use clean techniques during isolation and purification processes. There are several methods available for isolating RNA from various tissues, including {phenol-chloroform extraction, column chromatography, an